NCSU

BIOTECHNOLOGY TRAINING PROGRAM

BIT 815/815L DEEP SEQUENCING

SPRING 2012


                           
                                                                        Instructor: Dr. Jennifer Schaff                                                                 Course Format: One lecture and one lab per week
                                                                         Office: 2518 Thomas Hall, meetings by appointment                                         Lectures: Monday 3:20-5:30 pm
                                                                           Phone: 513-0738                                                                                           Labs: Friday 12:35-5:35 pm
                                                                           Email: jeschaff@ncsu.edu

                                                                        TA: Aaron Hawkins                                                                              TA: Cory Dashiell
                                                                           Email: abhawki2@ncsu.edu                                                                        Email: cldashie@ncsu.edu
                                                                          Office: 3309 Partners II                                                                                Office: Thomas Hall
                                                                          Office Hours: by appointment                                                                      Office Hours: by appointment
                                                                         

                                                                          Course Syllabus
                                                                          Lab Notebook Guidelines
                                                                          Lab Report Guidelines
                                                                          Lab Report info and Paper Presentations


                                          

Lecture Notes Lab Handouts
Week 1 Lecture 1 Lab 1 Protocol
Agarose Gel Electrophoresis
Qiagen MinElute Protocol
Qiagen Gel Extraction Protocol
Week 2 MLK Day - No lecture
Lab 2 - Protocol
Lab 2 - additional slides
Week 3 Lecture 2 Lab 3 - Protocol
Lab 3 - additional slides
Week 4 Lecture 3 Lab 4 - Protocol
Week 5 Lecture 4 Lab 5 - Protocol
Lab 5 - Alternate Protocol
Lab 5 - additional slides
Week 6 Lecture 5 Lab 6 - Protocol
Week 7 Lecture 6 Lecture 7